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Radical scavening, antimicrobial activity and phenolic content of Castanea Sativa extracts

Radical Scavenging and antimicrobial activity of extracts of three cultivar of Castanea Sativa Mill. have been examined. Leaf and catkins have been extracted under the same conditions by 50% ethanol as extragens, afterwards dry extracts of examined samples have been gained. Total phenolic content was determined by HPLC, subsequent to preliminary acid hydrolysis of samples. For determination of extract infl uence to creating and transformation of hydroxide (OH) radicals and (DPPH) radicals, extracts have been dissolved in distillated water and added to Fenton’ s model system in concentration of 0.2 mg/ml. High value antioxidant activity (AA) in relation to hydroxyl radicals has turned out catkins from Lovran chestnut (59.09%). The greatest antiradical activity in relation to DPPH radicals occurred with extracts of catkins from domestic sweet chestnut (37.50). The antimicrobial tests have showed that ethanol extracts of leaf and catkins inhibited growth of Gram positive bacteria.

antioxidant activity ; antimicrobial activity ; Castanea sativa ; extract ; HPLC ; EPR spectrometry

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