engleski

Perforin-expressing lymphocytes in peripheral blood and decidua of human first-trimester pathological pregnancies

Problem: We have shown previously that the decidua of first trimester human pregnancy is heavily infiltrated with perforin-positive cells. The aim was to detect expression of perforin in both decidual lymphocytes (DL) and peripheral blood lymphocytes (PBL) in the first trimester of pathological pregnancies: Anembryonic pregnancy and missed abortion. Methods: Decidual tissue from normal pregnancy group and from pathological pregnancies was obtained by vaginal curettage. P (an intracellular antigen) and the cell surface antigens CD3, CD4, CD8, CD16, CD56, CD11c and CD45Ra were quantified simultaneously by flow cytometric analysis. Results: In the missed abortion group, we found: 1) a relative decrease in the frequency of both CD4+P+ cells and CD56+P+ cells as well as the mean fluorescence intensity for perforin, 2) a relative increase of CD16+P+ PBL cells and 3) a relative increase of CD4+ cells in PBL compared with anembryonic pregnancy and normal pregnancy. There was also a significant relative decrease in the proportion of CD4+ and CD8+ cells among perforin-positive PBL in both anembryonic pregnancy and missed abortion. Conclusion: Our results show that significant decreases in prevalence of perforin-positive lymphoid cells, their subpopulations and mean fluorescence intensity for perforin are associated with pregnancy failure.

decidua; normal pregnancy; pathological pregnancies; perforin

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