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Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia (CROSBI ID 554163)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Špičić, Silvio ; Cvetnić, Željko ; Pate, Mateja ; Duvnjak, Sanja ; Zdelar-Tuk, Maja ; Račić, Ivana Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia // ESM 30th Annual Congress, Porto, Portugal / Suzana David (ur.). Porto, 2009

Podaci o odgovornosti

Špičić, Silvio ; Cvetnić, Željko ; Pate, Mateja ; Duvnjak, Sanja ; Zdelar-Tuk, Maja ; Račić, Ivana

engleski

Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia

A total of 9 M. avium subsp. avium strains isolated from bovines (N=1), pigs (N=2), wild boars (N=2) and poultry (N=4) were genotyped by PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) analysis. The isolates were collected in the period from 2001 to 2006. Revealed profiles were designated according to the nomenclature established and used in the OIE reference laboratory for avian tuberculosis in Brno, Czech Republic. Digestion with restriction endonuclease PvuII resulted in 3 PvuII RFLP profiles (F, Q and M), comprising 8-9 bands. Digestion with restriction endonuclease PstI was successfully accomplished in 8 isolates demonstrating 4 different profiles of 11-13 bands. Among them, 3 were found to be new in the database (A31, A32 and A33). Combination of PvuII– PstI digestion revealed 4 RFLP profiles (A29/F, A31/F, A32/F, A33/M). No epizootiological connection was found between the isolates expressing the predominant profile (A29/F), found in pigs, wild boars and poultry. This is the first research in the field of genotyping M. avium subsp. avium strains isolated from different animal species in Croatia.

Mycobacterium avium subsp. avium; RFLP

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Podaci o prilogu

2009.

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objavljeno

Podaci o matičnoj publikaciji

ESM 30th Annual Congress, Porto, Portugal

Suzana David

Porto:

Podaci o skupu

European Society of Mycobacteriology 30th Annual Congress

poster

05.07.2009-08.07.2009

Porto, Portugal

Povezanost rada

Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina