Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia (CROSBI ID 554163)
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Podaci o odgovornosti
Špičić, Silvio ; Cvetnić, Željko ; Pate, Mateja ; Duvnjak, Sanja ; Zdelar-Tuk, Maja ; Račić, Ivana
engleski
Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia
A total of 9 M. avium subsp. avium strains isolated from bovines (N=1), pigs (N=2), wild boars (N=2) and poultry (N=4) were genotyped by PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) analysis. The isolates were collected in the period from 2001 to 2006. Revealed profiles were designated according to the nomenclature established and used in the OIE reference laboratory for avian tuberculosis in Brno, Czech Republic. Digestion with restriction endonuclease PvuII resulted in 3 PvuII RFLP profiles (F, Q and M), comprising 8-9 bands. Digestion with restriction endonuclease PstI was successfully accomplished in 8 isolates demonstrating 4 different profiles of 11-13 bands. Among them, 3 were found to be new in the database (A31, A32 and A33). Combination of PvuII– PstI digestion revealed 4 RFLP profiles (A29/F, A31/F, A32/F, A33/M). No epizootiological connection was found between the isolates expressing the predominant profile (A29/F), found in pigs, wild boars and poultry. This is the first research in the field of genotyping M. avium subsp. avium strains isolated from different animal species in Croatia.
Mycobacterium avium subsp. avium; RFLP
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Podaci o prilogu
2009.
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objavljeno
Podaci o matičnoj publikaciji
ESM 30th Annual Congress, Porto, Portugal
Suzana David
Porto:
Podaci o skupu
European Society of Mycobacteriology 30th Annual Congress
poster
05.07.2009-08.07.2009
Porto, Portugal