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Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia


Špičić, Silvio; Cvetnić, Željko; Pate, Mateja; Duvnjak, Sanja; Zdelar-Tuk, Maja; Račić, Ivana
Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia // ESM 30th Annual Congress, Porto, Portugal / Suzana David (ur.).
Porto, Portugal, 2009. (poster, nije recenziran, sažetak, ostalo)


Naslov
Typing of Mycobacterium avium subsp. avium from different sources using PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) in Croatia

Autori
Špičić, Silvio ; Cvetnić, Željko ; Pate, Mateja ; Duvnjak, Sanja ; Zdelar-Tuk, Maja ; Račić, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
ESM 30th Annual Congress, Porto, Portugal / Suzana David - Porto, Portugal, 2009

Skup
European Society of Mycobacteriology 30th Annual Congress

Mjesto i datum
Porto, Portugal, 05-08.07.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Mycobacterium avium subsp. avium; RFLP

Sažetak
A total of 9 M. avium subsp. avium strains isolated from bovines (N=1), pigs (N=2), wild boars (N=2) and poultry (N=4) were genotyped by PvuII– PstI– IS901 restriction fragment length polymorphism (RFLP) analysis. The isolates were collected in the period from 2001 to 2006. Revealed profiles were designated according to the nomenclature established and used in the OIE reference laboratory for avian tuberculosis in Brno, Czech Republic. Digestion with restriction endonuclease PvuII resulted in 3 PvuII RFLP profiles (F, Q and M), comprising 8-9 bands. Digestion with restriction endonuclease PstI was successfully accomplished in 8 isolates demonstrating 4 different profiles of 11-13 bands. Among them, 3 were found to be new in the database (A31, A32 and A33). Combination of PvuII– PstI digestion revealed 4 RFLP profiles (A29/F, A31/F, A32/F, A33/M). No epizootiological connection was found between the isolates expressing the predominant profile (A29/F), found in pigs, wild boars and poultry. This is the first research in the field of genotyping M. avium subsp. avium strains isolated from different animal species in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina



POVEZANOST RADA


Projekt / tema
048-0481153-1150 - Molekularna epizootiologija važnih bakterijskih zoonoza (Željko Cvetnić, )

Ustanove
Hrvatski veterinarski institut, Zagreb