engleski

Antitumor effect of natural selenium compounds on human pancreatic and colonic carcinoma cells

Studies performed so far on different human carcinoma cell lines, as well as numerous case-control and epidemiologic studies have given proof to the protective effects of selenium against cancer. However, the anticancer properties of selenium are site-specific. The aim of this work was to evaluate the cytotoxic effect of selenium against CaCo2 0human colon carcinoma cells and SW620 lymph node metastasis of colon carcinoma cell line. Three selenium compounds, seleno-DL-cystine (SEC), seleno-L-methionine (SEM) and sodium selenite (SES), were used. Initial number of cells was 1.5×104 and the cells were incubated for 72 hr with aforementioned Se compounds at 10, 100 and 1000 ľM Se concentrations. Cytotoxicity was measured by the MTT cell survival assay. In the present study decreased viabilities of both CaCo2 and SW620 cells were established following the treatment with SEC, SES and SEM. At 10ľM Se levels all three chemical forms exerted a more or less anticipated cytotoxic effect with viability decreases ranging from 22 to 37%. The other two levels of 100 and 1000 ľM Se did not precipitate an expected proportional rise in cytotoxic effect compared to 10 ľM, which warrants further research on these cell lines. Nevertheless, the results confirm the applicability of Se in the prevention and treatment of the investigated cancer sites and this property of the element could be used in the development of new functional foods with Se as an active component.

selenium; cancer cells; cytotoxicity

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