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Pregled bibliografske jedinice broj: 42360

Evaluation of DNA Damage in Subjects Occupationally Exposed to Pesticides Using Single-Cell Gel Electrophoresis (SCGE) Assay


Garaj-Vrhovac, Vera; Želježić, Davor
Evaluation of DNA Damage in Subjects Occupationally Exposed to Pesticides Using Single-Cell Gel Electrophoresis (SCGE) Assay // Muatation Research, 469 (2000), 2; 279-285 (međunarodna recenzija, članak, znanstveni)


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Naslov
Evaluation of DNA Damage in Subjects Occupationally Exposed to Pesticides Using Single-Cell Gel Electrophoresis (SCGE) Assay

Autori
Garaj-Vrhovac, Vera ; Želježić, Davor

Izvornik
Muatation Research 469 (2000), 2; 279-285

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA damage; comet assay; human biomonitoring; pesticides

Sažetak
The comet assay, also called the single-cell gel electrophoresis (SCGE) is a rapid and sensitive method for the detection of DNA damage (strand breaks and alkali-labile sites) in individual cells. The assay is based on the embedding of cells in agarose, their lysis in alkaline buffer and finally subjection to an electric current. In the present study, alkaline single-cell gel electrophoresis was used to evaluate the extent of primary DNA damage and DNA repair in peripheral blood lymphocytes of subjects employed in pesticide production. After the period of high pesticide exposure lymphocytes of the occupationally exposed subjects manifested increased tail length and tail moment compared with the control group. After the workers spent next 6 months out of the pesticide exposure zone both endpoints were still increased in comparison to the control but significantly decreased in comparison to results of the first sample analysis.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
00220107

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Vera Garaj-Vrhovac (autor)

Avatar Url Davor Želježić (autor)

Citiraj ovu publikaciju

Garaj-Vrhovac, Vera; Želježić, Davor
Evaluation of DNA Damage in Subjects Occupationally Exposed to Pesticides Using Single-Cell Gel Electrophoresis (SCGE) Assay // Muatation Research, 469 (2000), 2; 279-285 (međunarodna recenzija, članak, znanstveni)
Garaj-Vrhovac, V. & Želježić, D. (2000) Evaluation of DNA Damage in Subjects Occupationally Exposed to Pesticides Using Single-Cell Gel Electrophoresis (SCGE) Assay. Muatation Research, 469 (2), 279-285.
@article{article, year = {2000}, pages = {279-285}, keywords = {DNA damage, comet assay, human biomonitoring, pesticides}, journal = {Muatation Research}, volume = {469}, number = {2}, title = {Evaluation of DNA Damage in Subjects Occupationally Exposed to Pesticides Using Single-Cell Gel Electrophoresis (SCGE) Assay}, keyword = {DNA damage, comet assay, human biomonitoring, pesticides} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts
  • Index Medicus





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