engleski

Antifungal and antimycotoxigenic properties of newly synthesized derivatives of coumarine

The effect of newly synthesized derivative of 11-hydroxy-7-imino-7H-7a, 12-diazbenzo/alfa/anthracen-6-one, on growth and aflatoxin B1 and G1 (AFB1 and AFG1) accumulation by toxigenic mold Aspergillus parasiticus NRRL 2999 was studied in yeast extract sucrose (YES) medium to determine the possible use of this compound as a mean of controlling aflatoxin accumulation. Experiments were carried out in a stationary culture at temperature of 28o C during 21 days. Mycelial dry weight were determined gravimetrically, and concentrations of AFB1 and AFG1 were measured by HPLC method using Hewlett-Packard instrument with fluorescence detector. Concentrations of investigated compound of 0.05 mmol x L-1 and 0.1 mmol x L-1, respectively, stimulated mold growth and AFB1 accumulation, but concentrations higher than 0.2 mmol x L-1 produced an inhibitory effect. In the presence of 0.3 mmol x L-1 of investigated coumarine, mold growth was decreased by 55% and toxin concentration by 85% or completely.

Molds; mycotoxins; antifungal agents; coumarines

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