engleski

Hsp70, TNFalpha and hsp32 gene polymorphisms and COPD

Genes encoding members of the heat shock protein (Hsp) 70 family, hsp70-1, hsp70-2 and hsp-hom, and gene encoding tumor necrosis factor alpha (TNFα ) lie in the class III region of the human major histocompatibility complex (MHC). Hsp70-1 and hsp70-2 genes encode the major heat and stress inducible Hsp70. Hsp70-hom encodes a protein that is highly related to Hsp70-1, but is not heat inducible. TNFα encodes pro-inflammatory cytokine, which plays an important role in inflammatory processes. Hsp32 gene (HOX-1) encodes inducible heme oxygenase-1, enzyme that catalyzes the degradation of heme, providing cellular protection against oxidative mediated injury. Polymorphisms of stress (hsp70 family and hsp32) and tumor necrosis factor alpha (TNFα ) genes have been reported to be related to chronic obstructive pulmonary disease (COPD), a disease associated with an immune as well as an oxidant/anti-oxidant imbalance. Therefore hsp70-2 A/G (+ 1267), hsp70-hom T/C (+ 2437) and TNFα G/A (+489) gene polymorphisms were analysed in patients with COPD by PCR-RFLP procedure. Polymorphisms of the HOX-1 gene regarding to the number of GT repeats in 5’ -flanking region were analysed with sequencing of PCR products. The obtained data showed that there were no statistically significant differences in the genotype distributions of hsp70-2 (Chi square test, P = 0, 186), hsp70-hom (Chi square test, P = 0, 859) and TNFα (Chi square test, P = 0, 299) between patients (31) with COPD and control subjects (48). Likewise there was no statistically significant difference in number of GT repeats in HOX-1 gene between patients with COPD and control subjects (Chi square test, P = 0, 907).

Hsp70-2; hsp70-hom; TNFα; hsp32; polymorphism; COPD

nije evidentirano