engleski

Affect of Molds Growth in Mixed Cultures on Production of Aflatoxins on Maize Hybrid

The relationship between mold biomass and the biosynthesis of aflatoxins B1 and G1 (AFB1 and AFG1) on solid substrates (whole and crushed maize grains) at temperatures from 15-40 oC and a water content in the substrate of 20-40% has been investigated.The experiments have been carried out with the aflatoxigenous mold Aspergillus flavus ATCC 26949 in pure and in mixed culture respectively, the latter with the common mold Trichothecium roseum ZMPBF 1226.T. roseum does not produce aflatoxins and chromatographically similar compounds.The amount of biomass was estimated by measuring the chitin content ; the aflatoxin concentration by means of UV light and ELISA method.It has been established that the biosynthesis of the examined aflatoxins and their ratio primarily depend on the temperature of cultivation, rather than on the growth of the mycelium.The biomass of the mixed culture of A. flavus and T. roseum after 42 days of cultivation reduces the amount of AFB1 by 20-45%, and the amount of AFG1 by 35-50% in respect to highest values obtained in the process of biosynthesis. The decrease of both toxins is more pronounced in the substrate at the higher water contents and the higher temperature of cultivation.The biomass of the pure culture of A. flavus after 42 days of cultivation reduces the amount of AFB1 by 15-25% and the amount of AFG1 by 25-35% at the same parameters of mold growth and of water content in the substrate.

Mixed mold cultures; Aflatoxins; Maize hybrid; ELISA method

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