engleski

Bile salts effect on surviving and morphology of potential probiotic strain Lactobacillus acidophilus M92

The important criterion in the selection of microbial strains for the probiotic use is a tolerance against bile salts in gastrointestinal tract. Consequently, surviving of the potential probiotic strain Lactobacillus acidophilus M92 was examined in the presence of conjugated and deconjugated bile salts. The bacterium L. acidophilus M92 did not grow, but survived in the presence of mixture conjugated bile salts, sodium-glycocholate and sodium-taurocholate, but grew in the presence deoxycholic acid. For L. acidophilus M92, conjugated forms of cholic and deoxycholic acid were more toxic than their deconjugated forms. Bile salts resistance was tested by monitoring of morphological characteristics of bacterial colonies and cells. In the original L. acidophilus M92 bacterial culture, cultivated in MRS medium smooth (S) and rough (R)appeared. It was established that smooth (S) colonies are more resistant than rough (R) colonies on bile salts effect. Inspection of single cells morphology from rough (R) colonies showed damages of cell wall caused by bile salts. This could mean that the colonial and cellular morphologies are valuble parameters in the selection of Lactobacillus strains for probiotic use.

bile salts; morphology; L. acidophilus

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