engleski

Design of species specific primers for amplification of mitochondrial DNA of Croatian wild animals

The goal of this paper was to design species specific primers for wolf (Canis lupus), fox (Vulpes vulpes), bear (Ursus arctos), roe deer (Capreolus capreolus), red deer (Cervus elaphus) and wild boar (Sus crofa). Primers have been designed using sequences of the listed species from the GenBank, using software BioEdit and Primer 3. We have defined the size and location of primers for each species, and size of the PCR product. With this species specific primers animal species can be identified using minimal quantities of different tissues using only DNA isolation, PCR and electrophoresis, so they can be used in a variety of ecological researches and in forensic cases.

primer; mitochondrial DNA; control region; wild animal

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