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Insulin administration in the mild hyperglycaemia changes expression of proinflammatory adhesion molecules on human aortic endothelial cells (CROSBI ID 153829)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Bačun, Tatjana ; Glavaš-Obrovac, Ljubica ; Belovari, Tatjana ; Mihaljević, Ivan ; Hanich, Toni ; Feher Belaj, Vesna, Včev, Aleksandar Insulin administration in the mild hyperglycaemia changes expression of proinflammatory adhesion molecules on human aortic endothelial cells // Collegium antropologicum, 34 (2010), 3; 911-915

Podaci o odgovornosti

Bačun, Tatjana ; Glavaš-Obrovac, Ljubica ; Belovari, Tatjana ; Mihaljević, Ivan ; Hanich, Toni ; Feher Belaj, Vesna, Včev, Aleksandar

engleski

Insulin administration in the mild hyperglycaemia changes expression of proinflammatory adhesion molecules on human aortic endothelial cells

An overexpression of cell adhesion molecules (CAMs) on the surface of endothelial cells is one of the first steps in a high glucose-mediated endothelial dysfunction in diabetic patients. The effect of insulin administration in the condition of elevated glucose concentration on the E-selectin, intracellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) expression on human aortic endothelial cells (HAEC) was investigated. Cells were cultured for 4 h in a medium supplemented with homocysteine (7 μ M) and different concentration of glucose (5.5, 8.0, 12.0 and 16.5 mM respectively) with or without insulin (1 mIU/mL) addition. Expression of CAMs was analysed by flow-cytometry using monoclonal antibodies. Controls were CAMs expression in the medium with a physiological glucose concentration (5.5 mM). Obtained results show that short-term exposure of HAECs to moderate high glucose concentrations results in increased expression of E-selectin (2-fold), VCAM-1 (3-fold) and ICAM-1 (47%). At the same time, HAEC grown with 12 mM glucose expressed lesser E-selectin and, more ICAM-1 (for 64%) and VCAM-1 (41%) molecules. 16.5 mM glucose decreased expression of all investigated adhesion molecules. Addition of insulin was not changed expression of CAMs expression in a medium with 5.5 mM glucose. In conditions of elevated glucose concentration (12 mM), addition of insulin significantly dropped E-selectin (27%) and increased VCAM-1 (23%) expression. In conclusion, moderate elevated glucose concentration increased expression of cell adhesion molecules on HAEC. Insulin administration in the mild hyperglycaemia reduces an expression of the proinflammatory adhesion molecule E-selectin which could contribute in deceleration of macrovascular complications development in diabetic patients.

cell adhesion molecules; expression; glucose-mediated endothelial dysfunction; insulin administration

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Podaci o izdanju

34 (3)

2010.

911-915

objavljeno

0350-6134

Povezanost rada

Kemija, Temeljne medicinske znanosti, Biologija

Poveznice
Indeksiranost