Proteomics as a Tool for Characterization of Therapeutic proteins from Human Plasma (CROSBI ID 552004)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Josić, Djuro ; Kovač, Spomenka ; Hixson, Douglas
engleski
Proteomics as a Tool for Characterization of Therapeutic proteins from Human Plasma
The application of proteomics technology in purification of proteins from human plasma and for characterization of plasma-derived therapeutics has been recently discussed. However, until now, the impact of this technology on the plasma protein fractionation and analysis of the final product has not been realized. In the presentwork, we demonstrate the use of proteomic techniques the monitoring of the first step of the plasma fractionation by use of anion-exchange chromatography. This chromatographic method is frequently used in the purification scheme for isolation of vitamin K dependent clotting factors II, VII, IX and X, and clotting inhibitors protein C and protein S, as well as inter-alpha inhibitor proteins (IaIp). After the removal of immunoglobulin G and non-binding proteins in the flow-through fraction, albumin and weakly bound proteins were eluted with low concentration of sodium chloride. The proteins that strongly bind to the anion-exchange column were eluted by higher salt concentrations. The fractions of interest were analyzed, and proteins were identified by LC– ESI-MS/MS. By use of this method, not only candidates for therapeutic concentrates, but also some potentially harmful components were identified. This strategy was very helpful for further process optimization, fast identification of target proteins with relatively low abundance, and for the design of subsequent steps in their removal or purification
Human plasma proteins
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Podaci o prilogu
6-6.
2008.
objavljeno
Podaci o matičnoj publikaciji
14th International Symposium on Separation Science, Book of Abstracts
Primošten:
Podaci o skupu
14th International Symposium on Separation Science
ostalo
30.09.2008-03.10.2008
Primošten, Hrvatska