engleski

Drug-resistant human laryngeal carcinoma cells have increased levels of cathepsin B

In our previous work we have shown the drug-resistance of cervical carcinoma, laryngeal carcinoma and glioblastoma cells may be accompanied by increased levels of tumor markers for invasion and metastasis ( i. e. urokinase type plasminogen activator, plasminogen activator inhibitor type 1, and/or cathepsin D). In the present study we examined the concentration of cathepsins B, L and H in three drug-resistant clones isoloated from human laryngeal carcinoma (HEp2). The basal levels of cathepsins B, L and H were determined by enzyme linked immunoabsorbent assay (ELISA). Our results show that all three clones had increased level of cathepsin B ( in two clones almost 4 fold increase was determined). The level of cathepsin L was altered (increased) only in VK2 clone, while the levels of cathepsin H were similar in parental cells and drug-resistant clones. Thus, our results suggest that drug-resistance may be accompanied with increased level of cathepsins B, i. e. tumor associated protease, involved in invasion and metastasis.

drug-resistance; tumor cells; cathepsin B; cathepsin L; cathepsin H

nije evidentirano