Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 413115

Application of Laser Tweezers to Studies of Membrane– Cytoskeleton Adhesion


Raucher Drazen
Application of Laser Tweezers to Studies of Membrane– Cytoskeleton Adhesion // Methods in Cell Biology / John Correa and Dr. H. William Detrich, III (ur.).
USA: Academic Press, 2008. str. 451-466


CROSBI ID: 413115 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Application of Laser Tweezers to Studies of Membrane– Cytoskeleton Adhesion

Autori
Raucher Drazen

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Methods in Cell Biology

Urednik/ci
John Correa and Dr. H. William Detrich, III

Izdavač
Academic Press

Grad
USA

Godina
2008

Raspon stranica
451-466

ISBN
9780123725219

Ključne riječi
laser optical tweezers, membrane cell adhesion

Sažetak
Interaction between the plasma membrane and cytoskeleton is important in regulating cell functions such as lamellipodial extension, cell motility, and endocytosis. Here, we describe a method to determine plasma membrane-cytoskeleton adhesion by measuring tether force using laser optical tweezers. We show the basic components and instrumental setup of a laser optical tweezers system which is used to pull thin membrane tethers from the cell surface using one micron polystyrene beads coated with membrane binding ligands. We also show an application of tether force measurements in elucidating regulation of membrane-cytoskeleton adhesion. That phosphatidylinositol-4, 5-biphosphate (PIP2) may play an important role in regulating membrane-cytoskeleton adhesion is suggested by correlating the plasma membrane concentration of PIP2 with membrane-cytoskeleton adhesion using tether force measurements. The assay described here enables us to investigate mechanochemical components of many important cellular processes, such as lamellipodial extension, cell motility, membrane resealing after cell wounding, and endocytosis. Furthermore, this method could be used to establish the link between biochemical function and physical properties of the plasma membrane which drives these dynamic cellular processes.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
219-0000000-3364 - Ciljano toplinsko dopremanje lijekova u solidne tumore (Raucher, Dražen, MZOS - )

Ustanove
Medicinski fakultet, Osijek

Profili:

Avatar Url Dražen Raucher (autor)

Citiraj ovu publikaciju

Raucher Drazen
Application of Laser Tweezers to Studies of Membrane– Cytoskeleton Adhesion // Methods in Cell Biology / John Correa and Dr. H. William Detrich, III (ur.).
USA: Academic Press, 2008. str. 451-466
Raucher Drazen (2008) Application of Laser Tweezers to Studies of Membrane– Cytoskeleton Adhesion. U: John Correa and Dr. H. William Detrich, I. (ur.) Methods in Cell Biology. USA, Academic Press, str. 451-466.
@inbook{inbook, editor = {John Correa and Dr. H. William Detrich, I.}, year = {2008}, pages = {451-466}, keywords = {laser optical tweezers, membrane cell adhesion}, isbn = {9780123725219}, title = {Application of Laser Tweezers to Studies of Membrane and \#8211; Cytoskeleton Adhesion}, keyword = {laser optical tweezers, membrane cell adhesion}, publisher = {Academic Press}, publisherplace = {USA} }




Contrast
Increase Font
Decrease Font
Dyslexic Font