engleski

Cell penetrating peptides (CPPs) fused to Elastin -Like polypeptide (ELP) as anti-metastasis agents

Tumor cell metastasis is a complex, multi-step process that is a major cause of morbidity and death amongst cancer patients. Cell adhesion plays a critical role in the development of metastatic cancer, and it is mediated by interactions between receptors on the cell surface and ligands of the extracellular matrix or other surfaces. Therefore, inhibition of the cell adhesion process appears to be an effective method of preventing metastasis. To prevent cell adhesion, we developed genetically engineered polypeptides with the potential to inhibit metastases. We have found that the cell penetrating peptides (CPP) Tat or penetratin (Pen), fused with elastin-like polypeptide (CPP-ELP) inhibited adhesion, spreading, invasion and migration of SKOV-3 ovarian cancer cells, SK-MEL-2 melanoma cells, and MDA-MB-231 breast cancer cells in cell culture. Furthermore, we have also confirmed that Tat-ELP has anti-metastatic potential in an experimental ovarian cancer metastasis model in vivo. Therefore, these polypeptides have great potential as a therapeutic intervention in cancer metastasis.

ELP; targeted drug delivery; solid tumors

Molim ispraviti zapis prema uputi u zapisu 413036, hvala, admin (2009-09-03)