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Pregled bibliografske jedinice broj: 412977

Targeting a c-Myc Inhibitory Polypeptide to Specific Intracellular Compartments using Cell Penetrating Peptides


Bidwell, Gene L. 3rd; Davis, Aisha N.; Raucher, Dražen
Targeting a c-Myc Inhibitory Polypeptide to Specific Intracellular Compartments using Cell Penetrating Peptides // Journal of controlled release, 135 (2009), 1; 2-10 doi:10.1016/j.jconrel.2008.11.015 (međunarodna recenzija, članak, znanstveni)


Naslov
Targeting a c-Myc Inhibitory Polypeptide to Specific Intracellular Compartments using Cell Penetrating Peptides

Autori
Bidwell, Gene L. 3rd ; Davis, Aisha N. ; Raucher, Dražen

Izvornik
Journal of controlled release (0168-3659) 135 (2009), 1; 2-10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Elastin-like polypeptide; c-Myc; thermal targeting; cell penetrating peptide; subcellular localization

Sažetak
The therapeutic index of current anti-cancer chemotherapeutics can be improved by two major mechanisms: 1) developing drugs which are specifically toxic to the cancer cells and 2) developing methods to deliver drugs to the tumor site. In an attempt to combine these approaches, we developed a thermally responsive polypeptide inhibitor of c-Myc. This polypeptide is based on the thermally responsive Elastin-like polypeptide (ELP). When injected systemically, ELP-fused drugs will aggregate and accumulate at the tumor site where local hyperthermia is applied. ELP was fused to a peptide which blocks c-Myc/Max dimerization (H1), thereby inhibiting transcription activation by c-Myc (ELP-H1). In this study, the cellular uptake, intracellular distribution, and potency of the Pen, Tat and Bac cell penetrating peptides fused to ELP-H1 were evaluated. While Pen-ELP-H1 and Tat-ELP-H1 were localized in the cytoplasm, Bac-ELP-H1 localized to the nucleus in a subset of the cells and was the most potent inhibitor of MCF-7 cell proliferation. This data demonstrates that ELP can be targeted to the desired cellular compartment simply by choice of the CPP used, resulting in a more potent nuclear targeted c-Myc inhibitory polypeptide which may be beneficial in cancer therapy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
219-0000000-3364 - Ciljano toplinsko dopremanje lijekova u solidne tumore (Dražen Raucher, )

Ustanove
Medicinski fakultet, Osijek

Autor s matičnim brojem:
Dražen Raucher, (280455)

Citiraj ovu publikaciju

Bidwell, Gene L. 3rd; Davis, Aisha N.; Raucher, Dražen
Targeting a c-Myc Inhibitory Polypeptide to Specific Intracellular Compartments using Cell Penetrating Peptides // Journal of controlled release, 135 (2009), 1; 2-10 doi:10.1016/j.jconrel.2008.11.015 (međunarodna recenzija, članak, znanstveni)
Bidwell, G., Davis, A. & Raucher, D. (2009) Targeting a c-Myc Inhibitory Polypeptide to Specific Intracellular Compartments using Cell Penetrating Peptides. Journal of controlled release, 135 (1), 2-10 doi:10.1016/j.jconrel.2008.11.015.
@article{article, year = {2009}, pages = {2-10}, DOI = {10.1016/j.jconrel.2008.11.015}, keywords = {elastin-like polypeptide, c-Myc, thermal targeting, cell penetrating peptide, subcellular localization}, journal = {Journal of controlled release}, doi = {10.1016/j.jconrel.2008.11.015}, volume = {135}, number = {1}, issn = {0168-3659}, title = {Targeting a c-Myc Inhibitory Polypeptide to Specific Intracellular Compartments using Cell Penetrating Peptides}, keyword = {elastin-like polypeptide, c-Myc, thermal targeting, cell penetrating peptide, subcellular localization} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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