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Pregled bibliografske jedinice broj: 412935

Comparison of molecular assays for detection and typing of human papillomavirus


Koidl, Christoph; Božić, Michale; Hadžisejdić, Ita; Grahovac, Maja; Grahovac, Blaženka; Kranewitter, Wolfgang; Marth, Egon; Kessler, Harald H.
Comparison of molecular assays for detection and typing of human papillomavirus // American Journal of Obstetrics and Gynecology, 199 (2008), 2; E1-1 doi:10.1016/j.ajog.2008.03.005 (međunarodna recenzija, članak, znanstveni)


Naslov
Comparison of molecular assays for detection and typing of human papillomavirus

Autori
Koidl, Christoph ; Božić, Michale ; Hadžisejdić, Ita ; Grahovac, Maja ; Grahovac, Blaženka ; Kranewitter, Wolfgang ; Marth, Egon ; Kessler, Harald H.

Izvornik
American Journal of Obstetrics and Gynecology (0002-9378) 199 (2008), 2; E1-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA extraction; human papillomavirus; microarray;

Sažetak
The objective of the study was to compare the performance of 3 different extraction instruments in conjunction with 4 different amplification and detection kits for detection and typing of human papillomavirus (HPV) deoxyribonucleic acid (DNA). A total of 42 cervical swabs were investigated. HPV DNA was extracted on the 3 different instruments. Each of the extracts was then amplified, and HPV DNA amplification products were detected with 4 different kits. In 31 samples, HPV DNA was detected by both the Amplicor HPV test and the LINEAR ARRAY HPV genotyping test in conjunction with DNA extraction on the easyMAG instrument. In another 6 samples, only low-risk types were detected with the LINEAR ARRAY HPV genotyping test. After extraction on the easyMAG instrument, 32 samples tested positive when the PapilloCheck with the HotStarTaq DNA polymerase was used. Together with extraction on the easyMAG instrument, the Amplicor HPV test, the LINEAR ARRAY HPV genotyping test, and the new PapilloCheck with the HotStarTaq DNA polymerase provide comparable results allowing reliable and safe HPV diagnostics in the routine laboratory. Use of alternative assays may lead to an increase of invalid and divergent HPV typing results. Key words: DNA extraction, human papillomavirus, microarray, polymerase chain reaction, typing

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

Napomena
Art. no. 144.e1.



POVEZANOST RADA


Projekt / tema
062-0000000-3550 - Molekularni mehanizmi infekcije virusom hepatitisa C (Blaženka Grahovac, )

Ustanove
Medicinski fakultet, Rijeka

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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