Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA (CROSBI ID 152915)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Lv, W ; Chen, X ; Aumiler, Damir ; Xia, Andong
engleski
Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA
The intersystem crossing and isomerization dynamics of free-Cy3, Cy3-ssDNA, free-Cy5 and Cy5-ssDNA are obtained through simple analysis of rapid on/off blinking from single molecule fluorescence intensity time-traces and the fluorescence correlation spectroscopy (FCS). The on- and off-times observed in fluorescence time traces of single cyanine dyes are due to the formation of the triplet state and isomerization, where both the interaction with DNA and long central polymethine chain of cyanine dyes increase the barriers of isomerization, leading to long off-time. The results indicate that the single molecule fluorescence fluctuation together with the resulting second autocorrelation analysis are powerful methods for determining the triplet state and isomerization dynamics, which could be the simple techniques and complementary to other spectroscopic techniques, such as fluorescence decay measurement and laser flash photolysis to study the photophysical processes of complex molecules.
cyanine dyes; single molecule spectroscopy; on/off blinking; fluorescence correlation spectroscopy; FCS
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Podaci o izdanju
52 (8)
2009.
1148-1153
objavljeno
1006-9291
10.1007/s11426-009-0059-2