Structural aspects of thiol-specific spin labeling of human plasma low density lipoprotein (CROSBI ID 86702)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Kveder, Marina ; Pifat, Greta ; Vukelić, Bojana ; Pečar, Slavko ; Schara, Milan
engleski
Structural aspects of thiol-specific spin labeling of human plasma low density lipoprotein
A novel thiol-specific spin labeling procedure for the protein component (apoprotein B, apoB) of low density lipoproteins (LDLs) is presented. A methanethiosulfonate spin label was used to probe the free cysteine residues of apoB with electron paramagnetic resonance (EPR) spectroscopy. The results indicated that the spin labeled sites are predominantly buried in the LDL particle in two distinct environments that differ in their mobility restrictions. The suitability of thiol-specific labeling for the study of the stability and conformation of apoB was demonstrated in experiments with denaturing agents. The results presented in this work offer a new approach for the matching of EPR data with the primary structure of apoB.
low density lipoprotein ; electron paramagnetic resonance ; thiol spin labeling of apoprotein B
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Podaci o izdanju
57 (6)
2000.
336-343
objavljeno
0006-3525
1097-0282
10.1002/1097-0282(2000)57:6<336::AID-BIP30>3.0.CO;2-1