engleski

Early Onset of Psoriasis Vulgaris and Polymorphisms of VDR Gene

Aim: The aim of this investigation were to establish an association between ApaI, BsmI and TaqI restriction fragment length polymorphism (RFLP) at the VDR gene in psoriatics with early onset of disease and healthy controls. Patients and Methods: 70 psoriatics (35 F:35 M) were randomly recruited and 157 healthy controls (86 F:71 M) with no clinical evidence or family history of psoriasis.Genomic DNA was extracted from peripheral blood leukocytes and the VDR gene was amplified using a polymerase chain reaction (PCR). The RFLP were coded as Aa (ApaI), Tt (TaqI) or Bb (BsmI), where a uppercase letter signifies absence of the restriction site and a lowercase letter signifies presence of the site. Gained results were processed by statistical analysis. Results: results of this study show that the four more frequent genotypes in healthy controls were: BbAaTt (29.3%), BBAatt (14.7%), BBaatt (14%) and bbAATT (12.7%), and in psoriasis patients were: BbAaTt (33.6%), BBAatt (22.9%), BBaatt(16.4%), and BbAATt (10%). Analysis (Westfall-Young) of genotype effects have not shown significant difference in distribution of BsmI (p < 0.206), ApaI (p < 0.826) or TaqI (p < 0.939) genotype frequencies between healthy controls and psoriasis patients. Conclusion: The results of the present study showed no relationship between psoriasis and the ApaI, BsmI, TaqI RFLP VDR genotypes, but BT haplotype showed protective effect on PV with early onset. This obtained data suggests that the VDR gene could be one of some candidate genes implicated in the pathogenesis of psoriasis vulgaris.

Psoriasis; Calcitriol; Receptor; Polymorphism; Restriction Fragment Length; Croatia

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