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Effects of oil industry high density brines in miniaturized algal growth bioassay and Lemna test

Aquatic primary producers have been frequently used for toxicity evaluation of many pollutants. Duckweeds and algae have different levels of complexity and could complement each other in phytotoxicity bioassays. Saturated water solutions of CaCl2, CaBr2 and their 1:1 mixture are commonly used for pressure control in oil wells. The effects of these solutions on the green alga Chlorella kessleri and on the macrophyte Lemna minor were studied. The o.5%, 1.0% and 1.5% (v/v)concentrations of all tested solutions stimulated the growth of C. kessleri during later phase of experiment. CaBr2 in 2.0% (v/v) concentration inhibited the growth during the hole experiment, while CaCl2 and 1:1 mixture just at the beginning. In Lemna test 0.1% and 1.5% (v/v) concentrations of all tested solutions also caused the stimulation of growth at the later phase of experiment, while all three solutions in 2.0% concentration showed the inhibition of L. minor growth during the whole experiment.

Lemna minor; Chlorella kessleri; miniaturized algal growth bioassay; Lemna test; calcium chloride; calcium bromide

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