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Accumulation of the β 2-adrenergic agonist clenbuterol in mouse dark hair


Pleadin, Jelka; Gojmerac, Tihomira; Lipej, Zoran; Mitak, Mario; Novosel, Dinko; Perši, Nina
Accumulation of the β 2-adrenergic agonist clenbuterol in mouse dark hair // Archives of toxicology, 83 (2009), 11; 979-983 doi:0.1007/s00204-009-0446-7 (međunarodna recenzija, članak, znanstveni)


Naslov
Accumulation of the β 2-adrenergic agonist clenbuterol in mouse dark hair

Autori
Pleadin, Jelka ; Gojmerac, Tihomira ; Lipej, Zoran ; Mitak, Mario ; Novosel, Dinko ; Perši, Nina

Izvornik
Archives of toxicology (0340-5761) 83 (2009), 11; 979-983

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
β 2-adrenergic agonist; Clenbuterol; Residues; Abuse; ELISA; Dark hair; Mouse

Sažetak
The aim of the present study was to evaluate the suitability of dark hair as a matrix for determination of the β 2-adrenergic agonist clenbuterol residues using previously validated enzyme-linked immunosorbent assay (ELISA) as a screening method for its quantitative determination. The experimental group of mice (n=60) were treated with two different anabolic dosages of clenbuterol for 15 days, whereas the control group of animals (n=30) were left completely untreated. Hair samples were collected on days 0, 5, 10 and 15 of treatment. Validation of the ELISA analytical procedure showed good recovery (mean recovery 74%) with an acceptable intra-assay variation in individual measurements for all hair samples to which 5, 10 and 50 ng/g clenbuterol were added (CV <10%). Low blank levels of clenbuterol (2.4± ; ; 0.6 ng/g) were measured in hair of untreated mice, whereas significantly higher clenbuterol concentrations rising proportionally with the time of treatment were recorded in hair of mice treated with lower (6.5 mg/kg body mass) and higher (12.5 mg/kg body mass) dose of clenbuterol. The peak hair concentration of clenbuterol measured on the last day of treatment (day 15) was 1553.9 140.1 ng/g and 6248.3 589.4 ng/g in the lower and higher dose group, respectively. Study results clearly indicated dark hair as a pigmented tissue to have a high accumulation potential for clenbuterol residues, thus being the target matrix of choice for detection of clenbuterol abuse as an anabolic in meat production.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
048-0531854-0467 - Ostaci kemijskih kontaminanata u hrani: Farmakokinetika i toksikologija (Dražen Oraić, )

Ustanove
Hrvatski veterinarski institut, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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