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Assessing the Fast Micromethod as a tool in DNA integrity measurement


Jakšić, Željko
Assessing the Fast Micromethod as a tool in DNA integrity measurement // Progress in DNA Damage Research / Miura, Souta ; Nakano, Shouta (ur.).
New York: Nova Publishers, 2008. str. 349-370


Naslov
Assessing the Fast Micromethod as a tool in DNA integrity measurement

Autori
Jakšić, Željko

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Progress in DNA Damage Research

Urednik/ci
Miura, Souta ; Nakano, Shouta

Izdavač
Nova Publishers

Grad
New York

Godina
2008

Raspon stranica
349-370

ISBN
1604565829

Ključne riječi
DNA alkali-labile sites, DNA cross-links, DNA integrity, DNA strand breaks, Fast Micromethod®

Sažetak
Over the past decade the relatively new Fast Micromethod® for detecting DNA damage (strand breaks, alkali-labile sites and incomplete excision repair) and related DNA integrity in cell suspensions or tissue homogenates in single microplates has been developed. The technique is based on the ability of the specific fluorophore PicoGreen® to preferentially interact with high integrity DNA molecules, dsDNA, allowing direct fluorimetric measurement of fluorophore-dsDNA complex decay in alkaline conditions. This analytical technique allows simple and fast simultaneous analysis of DNA integrity in numerous, low DNA content samples. Research has confirmed that the Fast Micromethod® is applicable for the measurement of DNA integrity in not only human biological material for medical purpose, but also in marine invertebrates for genotoxicity assessment (biomonitoring) and the estimation of harmful effects in the ecosystem. This article focuses on a comprehensive description of the method and its uses, and offers detailed descriptions and insights into samples and chemicals preparation, fluorescence analysis and calculation of DNA integrity rate. The results and features of many provided studies using this method are discussed, in order to elucidate advantages and imperfections of this analytical technique and to highlight approaches that may allow its further development.

Izvorni jezik
Engleski

Znanstvena područja
Geologija



POVEZANOST RADA


Projekt / tema
098-0982705-2724 - Učinak zagađenja na programirane biosinteze morskih beskralješnjaka (Renato Batel, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Željko Jakšić (autor)

Citiraj ovu publikaciju

Jakšić, Željko
Assessing the Fast Micromethod as a tool in DNA integrity measurement // Progress in DNA Damage Research / Miura, Souta ; Nakano, Shouta (ur.).
New York: Nova Publishers, 2008. str. 349-370
Jakšić, Ž. (2008) Assessing the Fast Micromethod as a tool in DNA integrity measurement. U: Miura, S. & Nakano, S. (ur.) Progress in DNA Damage Research. New York, Nova Publishers, str. 349-370.
@inbook{inbook, author = {Jak\v{s}i\'{c}, \v{Z}.}, year = {2008}, pages = {349-370}, keywords = {DNA alkali-labile sites, DNA cross-links, DNA integrity, DNA strand breaks, Fast Micromethod®}, isbn = {1604565829}, title = {Assessing the Fast Micromethod as a tool in DNA integrity measurement}, keyword = {DNA alkali-labile sites, DNA cross-links, DNA integrity, DNA strand breaks, Fast Micromethod®}, publisher = {Nova Publishers}, publisherplace = {New York} }