engleski

Chemical, cytotoxic and genotoxic properties of wastewater from metal industry

In many countries there is a growing problem concerning environmental pollution from industrial wastewaters. Majority of wastewaters that are released into environment satisfy law regulative on limit values of hazardous substances in wastewater, but mixture of compounds can cause deleterious effects on biological system. In this study we investigated chemical composition and genotoxic effects of wastewater collected from five locations of the region of metal industry. For determination of chemical composition of wastewater we used the method of atomic absorption spectrophotometry (AAS). Mutagenic potential of wastewater was determined on Salmonella typhimurium strains. Cytotoxic effect and primary DNA damage of wastewater was measured on human laryngeal and cervical carcinoma cells. Compounds present in five wastewater samples do not exceed permissible limits. Nevertheless, higher concentrations of wastewaters caused cytotoxic effect on both cell lines, but HeLa cell line was more responsive to lower concentrations of wastewater. All samples caused primary DNA damage in dose-responsive manner. Based on results obtained, additional treatment of the wastewater prior to discharge is recommended.

wastewaters; metal industry; atomic absorption spectrophotometry (AAS); mutagenicity; genotoxicity

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