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Pregled bibliografske jedinice broj: 407949

Lack of stereospecific effects of isoflurane and desflurane isomers in isolated guinea pig hearts


Graf, Bernhard M.; Boban, Mladen; Stowe, David F.; Kampine, John P.; Bošnjak, Željko J.
Lack of stereospecific effects of isoflurane and desflurane isomers in isolated guinea pig hearts // Anesthesiology, 81 (1994), 1; 129-136 (međunarodna recenzija, članak, znanstveni)


Naslov
Lack of stereospecific effects of isoflurane and desflurane isomers in isolated guinea pig hearts

Autori
Graf, Bernhard M. ; Boban, Mladen ; Stowe, David F. ; Kampine, John P. ; Bošnjak, Željko J.

Izvornik
Anesthesiology (0003-3022) 81 (1994), 1; 129-136

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Anesthetics; volatile; desflurane; Isoflurane; stereoisomers; Animal; guinea pig; Heart; coronary flow; electrophyslology; Isolated; left ventricular pressure; oxygen consumption; perfused

Sažetak
Volatile anesthetics alter membrane channel proteins. It is controversial whether they act by nonspecifically perturbing lipid membranes or by directly binding to amphiphilic and usually stereoselective regions on channel macromolecules. Biologically relevant receptors are usually stereoselective. The stereochemical effect of Isoflurane and desflurane can be used as a pharmacologic tool to investigate whether these drugs bind to specific target sites. The specific optical isomers of isoflurane and desflurane were used to examine whether they produce any differential effects on electrical, mechanical, and metabolic function in isolated hearts. Isolated guinea-pig hearts were perfused with Krebs-Ringer's solution containing, in random order, both isomers of either isoflurane (n = 11) or desflurane (n = 6) for 10 min with a 15-min washout period. Either anesthetic was injected into a preoxygenated, sealed bottle of perfusate, which gave concentrations of 0.28 and 0.57 min for isoflurane and 0.48 and 0.88 mM for desflurane, which are equivalent to 1 and 2 MAC multiples. Both isomers of isoflurane and desflurane decreased left ventricular pressure, heart rate, and percent oxygen extraction and increased atrloventricular conduction time, coronary flow, and oxygen delivery. Each change was significantly different from control at each concentration, and these effects were greater with the high compared to the low concentration of each anesthetic. There was no significant difference between the (+)- and the (-)-isomers for either anesthetic for any measured or calculated variable. Also, the effects of the stereoisomers were similar to those of the racemic mixture. These data indicate that the optical isomers of Isoflurane and desflurane are equipotent, as assessed by their effects on cardiac function in isolated guinea-pig hearts. Although both agents may ultimately influence hydrophilic domains of the protein channels, their major cardiac effect appears to result either from global perturbation of the membrane lipids and/or an interaction at nonstereoselective sites on channels modulating cardiac anesthetic effects.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Autor s matičnim brojem:
Mladen Boban, (207836)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE