INFLUENCE OF TNFd MICROSATELLITE POLYMORPHISM ON THE OUTCOME OF HEMATOPOIETIC STEM CELL TRANSPLANTATION (CROSBI ID 550737)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Štingl, Katarian ; Grubić, Zorana ; Čečuk-Jeličić, Esma ; Žunec, Renata ; Labar, Boris ; Serventi-Seiwerth, Ranka ; Brkljačić-Kerhin Vesna
engleski
INFLUENCE OF TNFd MICROSATELLITE POLYMORPHISM ON THE OUTCOME OF HEMATOPOIETIC STEM CELL TRANSPLANTATION
Proinflammatory cytokines such as TNFα are released as a consequence of conditioning regimens performed prior to the hematopoietic stem cell transplantation (HSCT) and serum levels of TNFα have been shown to correlate with occurrence of posttransplantion complications such as acute GvHD. Previous studies have found an association between TNFα production and TNFd microsatellite polymorphism. The aim of the present study was to investigate the possible correlation between this polymorphism and the survival of patients. Eighty patients who underwent HSCT were included in the analysis. All of them received a transplant from a sibling identical for HLA-A, -B, and -DRB1 loci. The analysis of the TNFd microsatellite was performed using PCR amplification with specific primers followed by electrophoresis on a polyacrylamide gel in ALFexpress sequencer with the B cell lines from 10th Workshop as controls. The distribution of alleles at TNFd locus among the patients did not differ from the results obtained for healthy individuals tested in previous studies. The predominance of TNFd4 allele (53.8% and 56.6% respectively) was observed in both groups and the frequencies of all other alleles were in agreement. The patients were divided according to the survival at day 100 post HSCT. No statistically significant difference was observed between patients who survived longer than 100 days post HSCT (N=68) and those who have not (N=12) with respect to the distribution of allele and genotype frequencies at TNFd locus as well as to the percentage of homozygous individuals. No correlation with worse survival rates was observed for the presence of alleles with higher number of repeats as previously suggested. The only marginally significant difference was found for TNFd3 allele, which was increased among patients with worse survival. This finding is in agreement with previous reports
TNFd microsatellite; HSCT
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Podaci o prilogu
2008.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
22nd European Immunogenetics and Histocompatibility Conference
poster
01.04.2008-05.04.2008
Toulouse, Francuska