engleski

Evaluation of CD45+ cells kinetics in the blood of fattening chickens immunized with live or inactivated Newcastle disease vaccine

Since insight into the effects of Newcastle disease vaccination on cellular immune responses has been insuffi cient to date, in this study the lymphocytes dynamics in the blood of fattening g chickens inoculated against Newcastle disease with lyophilised live vaccine of the La Sota virus strain (experimental group E1) and/or inactivated vaccine produced from the same strain (experimental group E2) were monitored. Blood was treated with labelled antibody for the CD45+ leukocyte marker and examined by fl ow cytometry. In both experimental groups a statistically signifi cant increase (P<0.01) of CD45+ cells was observed in relation to the control group of chickens, and the increase was more intensive and faster in the chickens vaccinated with lyophilised live vaccine than it was in chickens vaccinated with inactivated vaccine. The group treated with live vaccine had a signifi cantly increased number of CD45+ cells 6, 12, and 24 hr, and 7, 14, 21 and 28 days after inoculation. But on the 14th day after vaccination, a signifi cantly increased number of CD45+ cells (P<0.05) was found in the chickens inoculated with inactivated vaccine. It can be concluded that the lyophilised live vaccine was more effi cient and cellular immunity was achieved sooner than with the inactivated vaccine during the observed period of 28 days after inoculation.

cellular immunity ; flow cytometry ; fattening chickens ; Newcastle disease vaccine

nije evidentirano