engleski

Chip-based nanoelectrospray multistage mass spectrometry in neuroglycolipidomics: high-throughput analysis of gangliosides in defined regions of fetal brain

Gangliosides (GGs), a large group of sialylated glycosphingolipids, are considered biomarkers of human brain development, aging, and certain diseases. Determination of individual GG components in complex mixtures extracted from human brain represents a fundamental prerequisite for correlating their specificity with the specialized function of each brain area. In context of modern glycomics, detailed investigation of GG expression and structure in human brain requires a continuous development and application of innovative methods able to improve the quality of data and speed of analysis. In this work, for the first time a high-throughput mapping and sequencing of gangliosides in human fetal brain was performed by a novel mass spectrometry (MS)-based approach developed recently in our laboratory. Three GG mixtures extracted and purified from different regions of the same fetal brain in 36th gestational week: frontal neocortex (NEO36), white matter of the frontal lobe (FL36) and white matter of the occipital lobe (OL36) were subjected to comparative high-throughput screening and multistage fragmentation by fully automated chip-based nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) MS. By this method in only a few minutes of signal acquisition over one hundred GG and asialo-GG species were detected and identified in three minutes. Obtained data revealed for the first time that differences in GG expression in human fetal brain are dependent rather on phylogenetic development than topographic factors. While a significant variation of GG distribution in NEO36 vs. FL36 was observed, no significant differences in GG expression in white matter of frontal vs. occipital lobe were detected. Additionally, the largest number of species was identified in NEO36, which correlates with the functional complexity of neocortex as the newest brain region. In the last stage of analysis, by MS2-MS3 molecular ion fragmentation at variable amplitudes, NEO36-associated GD1b isomer could be clearly discriminated. Present results indicate that the combination of fully automated chipESI with HCT MSn is able to provide ultra-fast, sensitive and reliable analyses of complex lipid-linked carbohydrates from which the pattern of their expression and structure in a certain type of bio-matrix can be determined.

gangliosides analysis; fetal human brain regions; HCT MSn

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