engleski

Cholesterol concentration in seminal plasma as a predictive tool for quality semen evaluation

The aim of this study was to investigate the relationship between lipid composition of bovine serum and seminal plasma, seasonality and semen quality. The experiment was carried out in two groups of Simmental breeding bulls: group I (age 2-4 years) and group II (age 5-10 years). Blood samples were collected from jugular vein and bovine semen was sampled with an artificial vagina once per season. Serum concentrations of total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triacylglycerols, nonesterified fatty acids (NEFA) and lipoprotein electrophoretic patterns were determined. Seminal plasma concentrations of total cholesterol, HDL-C and LDL-C were assayed. Serum concentration of triacylglycerols in young bulls was significantly higher in winter as compared with autumn, while serum NEFA concentration was significantly higher in autumn than in other seasons (P<0.05 both). Serum concentration of total cholesterol, LDL-C and LDL lipoproteins in older bulls was significantly higher in winter than in spring (P<0.05). Seminal plasma concentration of total cholesterol in young bulls was significantly higher in spring as compared with summer, whereas in older bulls it was statistically significantly higher in winter as compared with autumn samples (P<0.05 both). Sperm volume of both groups was significantly higher in summer as compared with autumn and winter (P<0.05). Sperm motility in young bulls was lowest in summer and differed significantly from the values recorded in other seasons. The changes observed in seminal plasma cholesterol concentration were associated with extracellular lipid utilization and appeared to be applicable as a biochemical marker of sperm quality.

Simmental bulls; serum; seminal plasma; cholesterols; seasonality

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