Production and optimisation of microbial lipase (CROSBI ID 473524)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Milin, Čedomila ; Giacometti, Jasminka ; Giacometti, Fabio
engleski
Production and optimisation of microbial lipase
Industrial enzymes can be extracted from a naturally occurring source by production from a microorganism in fermentation. Lipases (3.1.1.3) belong to the class of serine hydrolases that break down triglycerides to fatty acids and glycerol. The fungus Aspergillus niger has been known to produce triacylglyccerol lipase. The extracellular lipase was produced by separating the cells from the fermentation broth without agitation in temperature controlled enviroment. Various parameters like initial pH, incubation period, effect of various nitrogen and carbon sources were optimised. Following the lipase activity, the best physicochemical conditions were found. It was observod that 72 hrs incubation, pH 6.0 and 30°C were optimum for growth, and production of lipase. The Monod equation was used for controlling the supply of a nutrient and the effect of some carbon and nitrogen sources were found. In all cases, the maximal specific rate of growth, u max, was determined. Besides the prediction of activities thorough computer programming was performed.
production of lipase; Aspergillus niger; optimisation of nutrients; lipase activity
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
102-102.
1999.
objavljeno
Podaci o matičnoj publikaciji
IXth International Congress of Bacteriology & Applied Microbiology : abstracts ; IXth International Congress of Mycology : abstracts
Sydney: Tour Hosts
Podaci o skupu
International Congress of Bacteriology & Applied Microbiology (9 ; 1999) ; International Congress of Mycology (9 ; 1999)
poster
16.08.1999-20.08.1999
Sydney, Australija