engleski

Evaluation of genome damage and its relation to oxidative stress induced by glyphosate in human lymphocytes in vitro

In the present study we evaluated genotoxic and oxidative potential of glyphosate on human lymphocytes at concentrations likely to be encountered in residential and occupational exposure. Testing was done with and without metabolic activation (S9). Ferric-reducing ability of plasma (FRAP), thiobarbituric acid reactive substances (TBARS) and hOGG1 modified comet assay were used to measure glyphosate’ s oxidative potential and its impact on DNA. Genotoxicity was evaluated by alkaline comet and cytome assay applying centromere probes. Alkaline comet assay showed significantly increased tail length (20.39 µ ; m) and intensity (2.19 %) for 580 µ ; g/ml and tail intensity (1.88 %) at 92.8 µ ; g/ml. With S9, tail length increased for all concentrations, being significant at 3.5, 92.8 and 580 µ ; g/ml. Comparing hOGG1 results with and without S9, significant increase was observed for tail intensity at 2.91 and 580 µ ; g/ml. Without S9, frequency of micronuclei, nuclear buds and nucleoplasmic bridges slightly increased from 3.5 µ ; g/ml. Presence of S9 significantly elevated cytome assay parameters only for 580 µ ; g/ml. FRAP values slightly increased only at 580 µ ; g/ml regardless of metabolic activation, while TBARS values increased significantly. Since for any of the assays applied, no clear dose-dependent effect was observed, it indicates that glyphosate in concentrations relevant to human exposure do not pose significant health risk.

hOGG1 comet assay; centromere; glyphosate; genotoxicity; metabolic activation; thiobarbituric acid reactive substances (TBARS)

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