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Pregled bibliografske jedinice broj: 403831

Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs : A multi-biomarker approach


Kopjar, Nevenka; Garaj-Vrhovac, Verica; Kašuba, Vilena; Rozgaj, Ružica; Ramić, Snježana; Pavlica, Vesna; Želježić, Davor
Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs : A multi-biomarker approach // International journal of hygiene and environmental health, 212 (2009), 4; 414-431 doi:10.1016/j.ijheh.2008.10.001 (međunarodna recenzija, članak, znanstveni)


Naslov
Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs : A multi-biomarker approach

Autori
Kopjar, Nevenka ; Garaj-Vrhovac, Verica ; Kašuba, Vilena ; Rozgaj, Ružica ; Ramić, Snježana ; Pavlica, Vesna ; Želježić, Davor

Izvornik
International journal of hygiene and environmental health (1438-4639) 212 (2009), 4; 414-431

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cytotoxic drugs; occupational exposure; genome damage; comet assay; cytogenetic endpoints

Sažetak
The aim of the present study was to evaluate genome damage induced in peripheral blood lymphocytes of Croatian health care workers occupationally exposed to cytotoxic drugs. A comprehensive multi-biomarker approach using the alkaline comet assay and cytogenetic endpoints (analysis of structural chromosome aberrations, SCE assay, lymphocyte proliferation kinetics and cytokinesis-block micronucleus assay) was employed. The study included two populations of subjects: 50 health care workers occupationally exposed to cytotoxic drugs and 50 control subjects matched in age, gender and smoking habit. An investigation regarding the handling practice with cytotoxic drugs was conducted in parallel. Results obtained indicate high exposure levels at workplace that should be reduced. The values recorded among the occupationally exposed subjects were as follows: mean comet tail length: 17.4670.08 mm ; the incidence of long-tailed nuclei: 54.6873.93% ; 4.4870.33 structural chromosome aberrations per 200 cells ; 5.8170.04 SCE per 50 cells ; 29.2872.21% of high-frequency cells ; proliferation rate index: 1.9770.12 ; and 16.3270.85 micronuclei per 1000 binuclear cells. All these values indicated higher levels of DNA and cytogenetic damage compared to the general population. Obtained results also confirmed that the frequency of long-tailed nuclei in the alkaline comet assay represents a helpful complement to other well-established comet parameters. The age of subjects and smoking habit significantly influenced the values of both comet and cytogenetic endpoints. Overall results of this study confirmed that handling cytotoxic drugs without appropriate safety precautions involves a potential genotoxic risk for exposed subjects. Before a strict monitoring of exposure levels on each workplace becomes a standard practice in Croatian hospitals, cytogenetic surveillance of exposed workers is also recommended, at least in cases of accidental exposure.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Vera Garaj-Vrhovac, )
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Klinika za tumore

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • CAB Abstracts
  • EMBASE (Excerpta Medica)
  • MEDLINE


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