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Odredivanje L-askorbinske kiseline elektrokemijskim senzorom s imobiliziranom tirozinazom


Mikšaj, Miroslav; Filipović-Kovačević, Željka; Grabarić, Zorana; Bratković, Nenad
Odredivanje L-askorbinske kiseline elektrokemijskim senzorom s imobiliziranom tirozinazom // XXI. Hrvatski skup kemičara i kemijskih inženjera – Knjiga sažetaka / Novak, Predrag (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa, 2009. (poster, nije recenziran, sažetak, znanstveni)


Naslov
Odredivanje L-askorbinske kiseline elektrokemijskim senzorom s imobiliziranom tirozinazom
(Tyrosinase Enzyme-Biosensor For L-Ascorbic Acid Determination)

Autori
Mikšaj, Miroslav ; Filipović-Kovačević, Željka ; Grabarić, Zorana ; Bratković, Nenad

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXI. Hrvatski skup kemičara i kemijskih inženjera – Knjiga sažetaka / Novak, Predrag - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa, 2009

ISBN
978-953-6894-38-3

Skup
XXI. Hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Trogir, Hrvatska, 19-22.04.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
L-askorbinska kiselina; tirozinaza; amperometrijski biosenzor
(L-ascorbic acid; tyrosinase; amperometric biosensor)

Sažetak
Tyrosinase is a copper-containing enzyme which catalyzes, in the presence of molecular oxygen two reactions: the transformation of o- mono-phenols into catechols and the oxidation of catechol to o- quinone. Various inhibitors of tyrosinase are know so as cyanide, herbicides and phenolic compounds. The inhibitory action of L-Ascorbic acid on enzymatic activity of tyrosinase have been studied. An amperometric biosensor based on enzyme kinetics of tyrosinase for L-Ascorbic acid was developed. Graphite electrode was applied as transductor. The biosensor was constructed by using gelatin or CSA and crosslinking agent glutaraldehyde to immobilise tirosinase (Mushroom tyrosinase) on carbon disk electrode. Electrode surface was previously modified with K2NiII[FeII(CN)6] in order to catalize the oxidation or reduction of solute species electrocatalytic activity. Measurements are generally carried out by Flow-injection analisyis (FIA). The proposed electrochemical procedure was successfully applied to determination of vitamin C in soft drinks and fruit juces. A detection limit of 4.5· ; 10– 5 mol L– 1 was obtained. The results were compared by a reference spectrophotometric method.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0582261-2253 - Ekološke priprave i elektrokemijska karakterizacija biološki aktivnih spojeva (Marijana Jukić, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb