engleski

Fatty acids and polyphenols in tissue regeneration and reparation

This project aims to identify the effects of fatty acids and polyphenols on the: - tissue regeneration and tissue reparation processes (in the model of the liver regeneration induced by partial hepatectomy) - toxic damage (in the model of tissue intoxication provoked by the pesticide). For that purpose, we will test the influence of diet enriched with the: - olive oil (oleic acid-n9), - lard (oleic acid-n9 and saturated fatty acids) - corn oil (linoleic-n6 and linolenic acid-n3), and - pumpkin seed oil (linoleic-n6 and oleic acid-n9), as well as the - polyphenols extracted from the olive oil on the: - lipid status of the relevant organs (determination of free fatty acids, triglycerides, cholesterol, phospholipids) - SPE, GC and HPLC analysis, - the expression of the transcriptional factors (PPARα and NFκ B)- qPCR, - lipoxygenase and cyclooxygenase mediated arachidonic acid metabolic pathway (activity of LOX-5, expression of COX-2, inflammatory lipid mediator synthesis)- ELISA, LC-MS analysis, - antioxidant status (antioxidant enzyme assay) and lipid peroxidation parametars (HPLC analysis of MDA), - Phase I biotransformation enzymes (CYP1A, CYP2E1, CYP3A)- qPCR, HPLC, LC-MS analysis. In addition, the composition and qualitative characterisation of the polyphenol extract (HPLC, LC-MS) and the residue of the pesticide in tissues (HPLC) will be determined. We are looking for research partners/ cordinators : (1) with experience in HPLC and LC/MS analysis of plant bioactive compounds (namely that found in olive oil), lipid analysis (free fatty acids, triglycerides, cholesterol, phospholipids), including lipid peroxidation products (eicosanoids, MDA) and CYT P450 enzymes analysis, and (2) with experience /equipment in molecular biotechnology methods.

fatty acids; polyphenols; tissue regeneration

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