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Pregled bibliografske jedinice broj: 403421

Ganglioside GT1b protects human spermatozoa from hydrogen peroxide-induced DNA and membrane damage


Gavella, Mirjana; Garaj-Vrhovac, Vera; Lipovac, Vaskresenija; Antica, Mariastefania; Gajski, Goran; Nikica, Car
Ganglioside GT1b protects human spermatozoa from hydrogen peroxide-induced DNA and membrane damage // International journal of andrology, 33 (2010), 3; 536-544 doi:10.1111/j.1365-2605.2009.00962.x (međunarodna recenzija, članak, znanstveni)


Naslov
Ganglioside GT1b protects human spermatozoa from hydrogen peroxide-induced DNA and membrane damage

Autori
Gavella, Mirjana ; Garaj-Vrhovac, Vera ; Lipovac, Vaskresenija ; Antica, Mariastefania ; Gajski, Goran ; Nikica, Car

Izvornik
International journal of andrology (0105-6263) 33 (2010), 3; 536-544

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA damage • ganglioside • hydrogen peroxide • spermatozoa

Sažetak
We have reported previously that various gangliosides, the sialic acid containing glycosphingolipids, provide protection against sperm injury caused by reactive oxygen species (ROS). In this study, we investigated the effect of treatment of human spermatozoa with ganglioside GT1b on hydrogen peroxide (H2O2)-induced DNA fragmentation and plasma membrane damage. Single- cell gel electrophoresis (Comet assay) used in the assessment of sperm DNA integrity showed that in vitro supplemented GT1b (100 μ m) significantly reduced DNA damage induced by H2O2 (200 μ m) (p < 0.05). Measurements of Annexin V binding in combination with the propidium iodide vital dye labelling demonstrated that the spermatozoa pre- treated with GT1b exhibited a significant increase (p < 0.05) in the percentage of live cells with intact membrane and decreased phosphatidylserine translocation after exposure to H2O2. Flow cytometry using the intracellular ROS-sensitive fluorescence dichlorodihydrofluorescein diacetate dye employed to investigate the transport of the extracellularly supplied H2O2 into the cell interior revealed that ganglioside GT1b completely inhibited the passage of H2O2 through the sperm membrane. These results suggest that ganglioside GT1b may protect human spermatozoa from H2O2- induced damage by rendering sperm membrane more hydrophobic, thus inhibiting the diffusion of H2O2 across the membrane.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Vera Garaj-Vrhovac, )
045-0000000-0174 - Humana reprodukcija-biološki i biofizički čimbenici funkcije membrane spermija (Mirjana Gavella, )
098-0982913-2332 - Molekularne interakcije u diferencijaciji limfocita (Mariastefania Antica, )
098-0982913-2858 - Regulacija dinamike citoskeleta u kretanju i diobi stanica (Igor Weber, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Klinika za dijabetes, endokrinologiju i bolesti metabolizma Vuk Vrhovac,
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • BIOSIS Previews (Biological Abstracts)
  • CAB Abstracts


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