engleski

Polymorphisms in the Interleukin-12 and IL-18 Genes and Recurrent Spontaneous Abortion

IL-12/IL-18 are involved in uNK control of uterine vascular development. Lower doses of IL-12/IL-18 are required for successful embryo development, while lack or high values of IL-12/IL-18 induce defects in uterine vascular remodeling during implantation and lead to pregnancy failure. Polymorphisms in the IL-12/IL-18 genes could modify the cytokine balance which might result in an increased susceptibility to recurrent spontaneous abortion (RSA). A case-control study was conducted to determine the association between the IL12 and IL18 (-607C>A, -137G>C) gene polymorphisms and the risk of RSA in 125 women with RSA and 136 controls. Polymerase chain reaction was performed to analyze genomic DNA for IL12B promoter insertion/deletion polymorphisms and IL18 (positions -607C>A, -137G>A) gene polymorphisms. The frequencies of CC, CA, AA genotypes for IL-18(-607) were: 34.4%, 54.4% and 11.2% in patients versus 30.1%, 58.1% and 11.8% in controls ; the frequencies of GG, GC, CC genotypes for IL-18(-137) were: 47.2%, 43.2% and 9.6% respectively in patients and 45.6%, 46.3% and 8.1% in controls ; the frequencies of DD, ID, II for IL-12 were, 25.6%, 52.8% and 21.6% in patients versus 21.3%, 51.5% and 27.2% in controls. When following recessive model for risk genotypes the resuls were as follows: for IL-18(-607 AA) OR=1.05, p=0.89 ; for IL-18 (-137 CC) OR=1.21, p=0.46 ; and for IL-12 (DD) OR=1.26, p=0.41). We found no significant association between IL-12 (ins/del) and IL-18 (-607 and -137) gene promoter polymorphisms and susceptibility to RSA in studied women.

recurrent spontaneous abortion susceptibility; genetic polymorphisms; IL-12; IL-18

doi: 10.1111/j.1600-0897.2007.00495.x