RecBCD enzyme in UV-irradiated Escherichia coli interferes with site-specific and general recombination of lambda prophage (CROSBI ID 473407)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Vlahović, Ksenija ; Zahradka, Davor ; Petranović, Mirjana
engleski
RecBCD enzyme in UV-irradiated Escherichia coli interferes with site-specific and general recombination of lambda prophage
RecBCD enzyme is involved in the radiation-induced process known as prophage inactivation. The process leads to the inability of lambda prophage to excise itself from the E. coli chromosome via site-specific recombination. In this work we wanted to further characterize the role of RecBCD enzyme in this process. In addition, we examined the ability of irradiated prophage to recombine with the infecting homologous phage. We used several E. coli mutants differentially altered in RecBCD's activities. The results showed that in the mutants carrying either recB2109 or recD1903, which do not exhibit significant nuclease activities, the prophage progressively loses its capacity for both site-specific and general recombination. In the recB268 null mutant, however, prophage recombinogenicity remained fully preserved. We also showed that the prophage unable to recombine retained its ability to complement the mutant infecting phage and that the recombination frequencies in phage x phage crosses were not affected by postirradiation incubation. Our results suggest that the helicase activity of RecBCD is responsible for the progressive loss of prophage recombinogenicity. This loss is most probably a consequence of the unsuccessful RecBCD-dependent recombinational repair of double-stranded breaks in the cell chromosome, during which some structures unsuitable for further recombination reactions may be produced.
Escherichia coli; lambda prophage; DNA recombination; recombinational DNA repair; RecBCD enzyme
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Podaci o prilogu
69-69-x.
2000.
objavljeno
Podaci o matičnoj publikaciji
Kongres hrvatskih biokemičara i molekularnih biologa HB2000 - Knjiga sažetaka
Floegel, Mirna
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu
Podaci o skupu
HB2000-Kongres hrvatskih biokemičara i molekularnih biologa
poster
13.10.2000-15.10.2000
Zagreb, Hrvatska