Naslov
Size-dependent palindrome-induced intrachromosomal recombination in yeast

Autori
Lisnić, Berislav ; Svetec, Ivan-Krešimir ; Štafa, Anamarija ; Zgaga, Zoran

Izvornik
DNA repair (1568-7864) 8 (2009), 3; 383-389

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
palindrome; SAE2; pop-out; genome stability; recombination

Sažetak
Palindromic and quasi-palindromic sequences are important DNA motifs found in various cis-acting genetic elements, but are also known to provoke different types of genetic alterations. The instability of such motifs is clearly size-related and depends on their potential to adopt secondary structures known as hairpins and cruciforms. Here we studied the influence of palindrome size on recombination between two directly repeated copies of the yeast CYC1 gene leading to the loss of the intervening sequence ("pop-out" recombination). We show that palindromes inserted either within one copy or between the two copies of the CYC1 gene become recombinogenic only when they attain a certain critical size and we estimate this critical size to be about 70 bp. With the longest palindrome used in this study (150 bp) we observed a more than 20-fold increase in the pop-out recombination. In the sae2/com1 mutant the palindrome-stimulated recombination was completely abolished. Suppression of palindrome recombinogenicity may be crucial for the maintenance of genetic stability in organisms containing a significant number of large palindromes in their genomes, like humans.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA