engleski

Wheat and maize MATH-BTB proteins are invoved in microtubule organization during the asymmetric cell division in female gametophyte and early embryo

By screening egg cell and proembryo cDNAs libraries of wheat and maize four genes encoding MATH and BTB domain proteins (TaMAB1-3 and ZmMAB1) have been identified. While only TaMAB3 is expressed constitutively, TaMAB1-2 and ZmMAB1 display an egg cell or zygote/proembryo specific expression pattern. TaMAB2 asymmetrically colocalises with microtubules at the nuclear envelope of BMS cells. Moreover, in transgenic Arabidopsis plants, TaMAB2 is always inherited to the basal cell of the proembryo. The interaction of TaMAB2 with AtCUL3 indicates its involvement in ubiquitin mediated proteosomal degradation. Downregulation of ZmMAB1 leads to an arrest of female gametophyte development due to the inability of correct microtubule associated spindle functions. The results provide strong evidence about a role of MABs as polarity factors involved in organisation of microtubules during female gametophyte development, zygotic division and early embryo formation, and perhaps asymmetric cell divisions in general.

MATH-BTB; megagametogehesis; embryogenesis

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