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Differences in Cholinesterases Affinities toward Chiral Quinuclidinium Compounds


Primožič, Ines; Rollinger, Judith Maria; Stuppner, Hermann; Hrenar, Tomica
Differences in Cholinesterases Affinities toward Chiral Quinuclidinium Compounds // XXI Hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka. / Novak, Predrag (ur.).
Kutina: Hrvatsko društvo kemijskih inženjera i tehnologa, 2009. str. 97-97 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Differences in Cholinesterases Affinities toward Chiral Quinuclidinium Compounds

Autori
Primožič, Ines ; Rollinger, Judith Maria ; Stuppner, Hermann ; Hrenar, Tomica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXI Hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka. / Novak, Predrag - Kutina : Hrvatsko društvo kemijskih inženjera i tehnologa, 2009, 97-97

ISBN
978-953-6894-38-3

Skup
XXI. hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Trogir, Hrvatska, 19.- 22.04.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Cholinesterases; chiral quinuclidinium compounds; kinetic studies; docking simulations

Sažetak
Non-quaternary and N-benzyl quaternary chiral quinuclidinium esters1, 2 and amides3, 4 of pivalic, butyric and benzoic acid were evaluated as substrates and/or inhibitors of cholinesterases. Inhibitory effect of quinuclidinium amides on acetylcholinesterase (AChE from electric eel) and butyrylcholinesterase (BuChE form horse serum) were determined using a spectrophotometric method (Ellman’ s reagent) in a microplate assay.5 Hydrolysis kinetics of chiral esters (HPLC analysis, substrate: benzoylcholine) revealed that (R)-enantiomers of tested esters are much better substrates than (S)-enantiomers. On the other hand, (S)-enantiomers of esters and amides showed higher affinity toward ChEs except in the case of the butyric acid derivatives. In order to explain experimental data concerning activity of enantiomers, molecular docking simulations were performed using AutoDock 4.0 suite of programs1. We employed flexible ligand docking (Lamarckian Genetic Algorithm (LGA) search method) allowing all possible torsions in molecules. The results are compared with those for benzoyl choline and galanthamine. It was shown that enantiomeric preference of cholinesterases, as well as rates of hydrolysis, are governed primarily by electrostatic interactions and steric limitations and in the choline subsite. The differences in orientation and relative energies will be presented and discussed.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
119-1191344-3121 - Sinteze i enzimske transformacije biološki aktivnih spojeva (Srđanka Tomić-Pisarović, )

Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju

Primožič, Ines; Rollinger, Judith Maria; Stuppner, Hermann; Hrenar, Tomica
Differences in Cholinesterases Affinities toward Chiral Quinuclidinium Compounds // XXI Hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka. / Novak, Predrag (ur.).
Kutina: Hrvatsko društvo kemijskih inženjera i tehnologa, 2009. str. 97-97 (poster, domaća recenzija, sažetak, znanstveni)
Primožič, I., Rollinger, J., Stuppner, H. & Hrenar, T. (2009) Differences in Cholinesterases Affinities toward Chiral Quinuclidinium Compounds. U: Novak, P. (ur.)XXI Hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka..
@article{article, editor = {Novak, P.}, year = {2009}, pages = {97-97}, keywords = {Cholinesterases, chiral quinuclidinium compounds, kinetic studies, docking simulations}, isbn = {978-953-6894-38-3}, title = {Differences in Cholinesterases Affinities toward Chiral Quinuclidinium Compounds}, keyword = {Cholinesterases, chiral quinuclidinium compounds, kinetic studies, docking simulations}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa}, publisherplace = {Trogir, Hrvatska} }