engleski

A royal jelly as a new potential immunomodulator in rats and mice

In order to study a possible immunomodulatory effect of the royal jelly (RJ) secreted by mandibular and hypopharingeal glands of the worker honeybee (Apis mellifera LINNÉ.) we have used well established rodent model. The CBA mice were given s/c 0.1 ml of RJ 7 days before or immediately after the immunization with sheep red blood cells (SRBC). The Y59 rats received i/m 0.4 ml or i/v 0.025 ml of RJ one to two times in 7 day intervals. Serum levels of total proteins and immunoglobulins in the rats that received RJ one to two times within 2-week-period were significantly lower (P Ł 0.05) as compared with the nontreated animals. In mice which were immunized with 4x10^8 of SRBC 7 days after the application of RJ the number of plaque forming splenocytes was significantly higher (P Ł 0.05) than that in the controls. Both, the weight of inguinal lymph node and the number of peripheral blood lymphocytes were increased (P Ł 0.05) in RJ-treated mice 3 or 5 days after the immunization, respectively. Neutrophils were decreased (P Ł 0.05) in the mice that were killed 5 or 10 days after the RJ treatment. Overall these results indicate that RJ exhibited immunomodulatory properties by stimulating the antibody production and the immunocompetent cell proliferation in mice or depressing humoral immune functions in rats. Both phenomena, though species-related in this model, could be probably reversed by changing the dose or the route of RJ application.

Immunomodulation; royal jelly; rat; mouse

nije evidentirano