engleski

The effect of tretment with ochratoxin A on oxidative stress in rat liver and kidney

Humans all round the World are continuously exposed to mycotoxin ochratoxin A (OTA) that frequently contaminates various foods and feeds. OTA is nephrotoxic, carcinogen and immunotoxic compound. It is known that the primary target organ of OTA toxicity in laboratory animals is kidney and that toxic effects are at least partially caused by ROS production and oxidative stress. The aim of this study was to find out whether OTA causes similar effects on parameters of oxidative stress in kidney and liver by measuring malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG) and glutathione (GSH). Male rats were treated orally by gavages with 0, 250 and 500 &#61549; g OTA/kg b.w. (five times, every other day), respectively and sacrificed 24 h after the last treatment. Urine samples were collected 24 hours before the beginning of the treatment and 24 hours after the last one. The concentrations of MDA and 8-OHdG were measured in urine, MDA and GSH in kidney and liver, and OTA in kidney, liver and urine. OTA, MDA and 8-OHdG were measured using HPLC with fluorescent, UV and electrochemical detectors, respectively, while GSH was measured spectrophotometrically. The concentrations of OTA in kidney, liver and urine, increased with the increase of applied doses. The concentration of MDA in kidney increased with the applied dose, but this increase was not significant. In contrast, the increase of MDA concentration in liver was significant (P<0.05). The concentrations of GSH in kidney and liver were not affected by OTA treatment. In urine, where the parameters of oxidative stress show the general oxidative status of the organism, neither MDA nor 8-OHdG concentrations in OTA treated animals were not different as compared to controls. Although OTA accumulated in kidney and liver and its concentration increased in urine, this did not affect significantly oxidative stress in kidney as measured with GSH and MDA concentrations. In contrast, in liver the increase in MDA concentration was parallel with the applied dose. Although it is known that the mechanism of OTA toxicity in kidney involves oxidative stress, our result indicate that this mycotoxins causes more severe lipid peroxidation in liver than in kidney.

glutathione; malondialdehyde; 8-hydroxy-2-deguanosine; nephrotoxicity

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