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Pregled bibliografske jedinice broj: 397591

Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection


Domijan, Ana-Marija; Peraica, Maja
Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection // Arhiv za higijenu rada i toksikologiju, 59 (2008), 277-282 doi:10.2478/10004-1254-59-2008-1879 (međunarodna recenzija, članak, znanstveni)


Naslov
Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection

Autori
Domijan, Ana-Marija ; Peraica, Maja

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 59 (2008); 277-282

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biomarker; free radicals; oxidative DNA damage; 8-oxodG

Sažetak
Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50-400 nM, the calibration curve was linear (r2= 0.9995) and the limit of detection was 3 nM. Concentrations of 8-oxodG ranged between 3.0 nM and 48.0 nM, which is in accordance with values reported for healthy individuals in other studies.

Izvorni jezik
Engleski

Znanstvena područja
Matematika, Kemija, Arhitektura i urbanizam



POVEZANOST RADA


Projekt / tema
022-0222148-2142 - Toksični učinci mikotoksina na ljude i životinje (Maja Peraica, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • MEDLINE


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