Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection (CROSBI ID 150662)
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Podaci o odgovornosti
Domijan, Ana-Marija ; Peraica, Maja
engleski
Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection
Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50-400 nM, the calibration curve was linear (r2= 0.9995) and the limit of detection was 3 nM. Concentrations of 8-oxodG ranged between 3.0 nM and 48.0 nM, which is in accordance with values reported for healthy individuals in other studies.
biomarker; free radicals; oxidative DNA damage; 8-oxodG
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Podaci o izdanju
59
2008.
277-282
objavljeno
0004-1254
10.2478/10004-1254-59-2008-1879
Povezanost rada
Kemija, Arhitektura i urbanizam, Matematika