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Pregled bibliografske jedinice broj: 396450

Articular Cartilage Repair by Genetically Modified Bone Marrow Aspirate in sheep


Ivković, Alan; Pascher, Arnulf; Hudetz, Damir; Matičić, Dražen; Jelić, Mislav; Dickinson, Sally C.; Loparić, Marko; Hašpl, Miroslav; Windhager, Reinhard; Pećina, Marko
Articular Cartilage Repair by Genetically Modified Bone Marrow Aspirate in sheep // Gene therapy, 17 (2010), 6; 822-833 doi::10.10338/gt.2010.16 (međunarodna recenzija, članak, znanstveni)


Naslov
Articular Cartilage Repair by Genetically Modified Bone Marrow Aspirate in sheep

Autori
Ivković, Alan ; Pascher, Arnulf ; Hudetz, Damir ; Matičić, Dražen ; Jelić, Mislav ; Dickinson, Sally C. ; Loparić, Marko ; Hašpl, Miroslav ; Windhager, Reinhard ; Pećina, Marko

Izvornik
Gene therapy (0969-7128) 17 (2010), 6; 822-833

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gene therapy ; articular cartilage repair ; bone marrow ; transforming growth factor beta1 ; cell transplantation

Sažetak
This study was performed to test whether abbreviated ex vivo protocol that utilizes vector-laden coagulated bone marrow aspirates for gene delivery to cartilage defects may be used in clinical application. Ovine autologous bone marrow was transduced with adenoviral vector containing cDNA for GFP or TGF-beta 1. The marrow was allowed to clot and then was transplanted into partial-thickness defect created on the medial condyle. At 6 months the quality of articular cartilage repair was evaluated using histological, biochemical and biomechanical parameters. The results of the repair assessment showed that the group treated with construct transplantation was better than the untreated controls. Improved cartilage repair was observed in groups treated with modified bone marrow clots and Ad.TGF-beta1 transduced clots, but the repaired tissue from TGF treated defects showed significantly higher amounts of collagen II. The results confirmed that proposed method is fairly simple and straightforward technique for application in clinical settings, and that genetically modified bone marrow clots are sufficient to facilitate cartilage repair in vivo.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Veterinarska medicina



POVEZANOST RADA


Projekt / tema
108-0000000-3652 - Genska terapija mineraliziranih tkiva (Marko Pećina, )
108-1080327-0161 - Liječenje bolesti i ozljeda hrskavice velikih zglobova (Miroslav Hašpl, )

Ustanove
Veterinarski fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Sveučilište Libertas

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • MEDLINE


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