engleski

Articular Cartilage Repair by Genetically Modified Bone Marrow Aspirate in sheep

This study was performed to test whether abbreviated ex vivo protocol that utilizes vector-laden coagulated bone marrow aspirates for gene delivery to cartilage defects may be used in clinical application. Ovine autologous bone marrow was transduced with adenoviral vector containing cDNA for GFP or TGF-beta 1. The marrow was allowed to clot and then was transplanted into partial-thickness defect created on the medial condyle. At 6 months the quality of articular cartilage repair was evaluated using histological, biochemical and biomechanical parameters. The results of the repair assessment showed that the group treated with construct transplantation was better than the untreated controls. Improved cartilage repair was observed in groups treated with modified bone marrow clots and Ad.TGF-beta1 transduced clots, but the repaired tissue from TGF treated defects showed significantly higher amounts of collagen II. The results confirmed that proposed method is fairly simple and straightforward technique for application in clinical settings, and that genetically modified bone marrow clots are sufficient to facilitate cartilage repair in vivo.

gene therapy ; articular cartilage repair ; bone marrow ; transforming growth factor beta1 ; cell transplantation

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