engleski

Epidural analgesia-induced immunomodulation of cell-mediated cytotoxicity in patients with colorectal carcinoma

Backgrouds and aims: The effect of postoperative pain management on immune function in patients with malignancies is a subject of a great scientific interest. Althought the suppression of immune response has been reported, we know very little about the cytotoxict function of freshly isolated NK cells of colorectal carcinoma patients underwent surgery and the effect of epidural postoperative analgesia. Immune compromise could affect the healing processes, postoperative infections and rate and size of tumor metastases disseminated during operation. The aim of this study was to correlate the intensity of these changes after epidural and intravenous postoperative analgesia with immunodisfunction, which usually occurs during the malignant process. Methods: Flow-cytometry cytotoxicity assay: the cytotoxicity of freshly isolated human mononuclear cells (MNLC) was tested against K-562 (NK- sensitive target). Test was made by the use of flow cytometry and PKH-26 Red Fluorescent Cell Linker Kit and 2h-cytotoxicity assay (Sigma Biosciences, St Louis, MO). Statistical analysis. Data were analyzed using the Sigma Plot Scientific Graphing System, Version 6.10. Linear regression equations were generated using a least-squares method and analyzed for differences of covariance. Statistical significance was calculated by Mann Whitney U test. The differences were considered significant for p 0.05. Results: The data have shown that epidural analgesia more than intravenous analgesia augmented decreased cytotoxicity of MNLC of colon carcinoma patients against K-562 targets. Conclusions: The data emphasize that during the postoperative pain management, epidural analgesia prevents some immune dysfunctions, contributing to the more efficient postoperative recovery than the patients undergoing intravenous analgesia. Acknowledgment: This work was supported by grants from the Croatian Ministry of Science (projects: 0620096- 0094 and 0620096-0092)

cytotoxicity; epidural postoperative analgesia; NK cells; NKT cells;

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