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Genetic potential, diversity and activity of an atrazine-degrading community enriched from a herbicide factory effluent


Udiković Kolić, Nikolina; Martin-Laurent, Fabrice; Devers, Marion; Petrić, Ines; Begonja Kolar, Ana; Hršak, Dubravka
Genetic potential, diversity and activity of an atrazine-degrading community enriched from a herbicide factory effluent // Journal of Applied Microbiology, 105 (2008), 5; 1334-1343 (međunarodna recenzija, članak, znanstveni)


Naslov
Genetic potential, diversity and activity of an atrazine-degrading community enriched from a herbicide factory effluent

Autori
Udiković Kolić, Nikolina ; Martin-Laurent, Fabrice ; Devers, Marion ; Petrić, Ines ; Begonja Kolar, Ana ; Hršak, Dubravka

Izvornik
Journal of Applied Microbiology (1364-5072) 105 (2008), 5; 1334-1343

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Atrazine; biodegradation; atz; trz; bacterial community; wastewater; quantitative PCR

Sažetak
Aims: To characterize an atrazine-degrading bacterial community enriched from the wastewater of a herbicide factory. Methods and Results: The community mineralized 81.4+/-1.9% of [14C-ring]atrazine and 31.0+/-1.8% of [14C-ethyl]atrazine within 6-days of batch cultivation in mineral salts medium containing atrazine as the sole nitrogen source. Degradation activity of the community toward different chloro- and methylthio-substituted s-triazine compounds was also demonstrated. Restriction analysis of amplified 16S rDNA (ARDRA) revealed high diversity of bacterial populations forming the community, with Pseudomonas species dominating in the clone library. Atrazine-degrading genetic potential of the community determined by PCR revealed the presence of trzN, atzB, atzC and trzD genes. The trzN, atzB, atzC genes were shown to be located on a plasmid of 322 kb. Quantitative PCR showed that relative abundances of atzB, atzC and trzD genes were approx. 100-fold lower than 16S rDNA. Conclusions: The enriched community represents a complex bacterial association expressing substantial atrazine mineralizing activity and a broad specificity toward a range of s-triazine compounds. Significance and Impact of the Study: Our study is beginning to yield insights into the richness, genetic potential and density of functional atrazine-mineralizing community that could be potential bioaugmentation agent for improving biotransformation processes in wastewaters bearing different s-triazine compounds.

Izvorni jezik
Engleski

Znanstvena područja
Geologija



POVEZANOST RADA


Projekt / tema
098-0982934-2712 - Organski spojevi kao molekulski obilježivači antropogenog utjecaja na okoliš (Marijan Ahel, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE