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Pregled bibliografske jedinice broj: 394353

Lindane-induced cytotoxicity and the role of vitamin E in Chinese hamster ovary (CHO-K1) cells


Kmetič, Ivana; Radošević, Kristina; Murati, Teuta; Šimić, Branimir; Kniewald, Zlatko; Kniewald, Jasna
Lindane-induced cytotoxicity and the role of vitamin E in Chinese hamster ovary (CHO-K1) cells // Toxicology mechanisms and methods, 19 (2009), 8; 518-523 doi:10.1080/15376510903280107 (međunarodna recenzija, članak, znanstveni)


Naslov
Lindane-induced cytotoxicity and the role of vitamin E in Chinese hamster ovary (CHO-K1) cells

Autori
Kmetič, Ivana ; Radošević, Kristina ; Murati, Teuta ; Šimić, Branimir ; Kniewald, Zlatko ; Kniewald, Jasna

Izvornik
Toxicology mechanisms and methods (1537-6524) 19 (2009), 8; 518-523

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CHO-K1 cells ; cytotoxicity ; IC50 values ; lindane ; vitamin E

Sažetak
Lindane, a toxic insecticide from the persistent organic pollutants (POP's) group, may act as an endocrine disrupter affecting crucial tissues of reproductive system. In this study a Chinese Hamster Ovary cell line (CHO-K1) was applied to assess the potential of lindane cytotoxicity at the cellular level. The methods of Trypan blue exclusion, MTT and Kenacid blue assays were used to assess cytotoxicity and confirmed a decrease in the number of viable CHO-K1 cells at 34.4-344 micro M lindane during 24, 48 and 72 hours of exposure. The cell proliferation tests showed significant inhibition (p<0.025-0.001 vs control) and a progressive increase in toxicity with increasing lindane concentrations. Corresponding IC50 values were determined with each applied method. After 72 h of lindane exposure, IC50 values were 184 micro M according to the Trypan blue method and 272 and 256 micro M with the Kenacid blue and MTT assays, respectively. Morphological changes induced by the cytotoxicity of lindane were followed by the fluorescence microscopy and only necrotic cells were detected. Vitamin E (25 and 50 micro g/mL) was used for protection of ovarian cells against lidane-induced oxidative stress damage, and lipid peroxidation was postulated as a possible mechanism of lindane toxicity. The viability of cells pre-incubated with vitamin E was significantly enhanced (up to p<0.025) compared to the results observed in cells exposed to lindane only, but vitamin E treatment could not prevent complete lindane-induced cytotoxicity. Results suggest that vitamin E may exert a slightly protective role in cell defense against lipophilic pro-oxidant xenobiotics such as lindane.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0582184-2232 - Endokrino-aktivne supstancije u hrani i oštećenja reproduktivnog sustava (Branimir Šimić, )
058-0582184-2414 - Nekliničke metode ispitivanja tvari iz okoliša na stanicama sisavaca i riba (Višnja Gaurina-Srček, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Citiraj ovu publikaciju

Kmetič, Ivana; Radošević, Kristina; Murati, Teuta; Šimić, Branimir; Kniewald, Zlatko; Kniewald, Jasna
Lindane-induced cytotoxicity and the role of vitamin E in Chinese hamster ovary (CHO-K1) cells // Toxicology mechanisms and methods, 19 (2009), 8; 518-523 doi:10.1080/15376510903280107 (međunarodna recenzija, članak, znanstveni)
Kmetič, I., Radošević, K., Murati, T., Šimić, B., Kniewald, Z. & Kniewald, J. (2009) Lindane-induced cytotoxicity and the role of vitamin E in Chinese hamster ovary (CHO-K1) cells. Toxicology mechanisms and methods, 19 (8), 518-523 doi:10.1080/15376510903280107.
@article{article, year = {2009}, pages = {518-523}, DOI = {10.1080/15376510903280107}, keywords = {CHO-K1 cells, cytotoxicity, IC50 values, lindane, vitamin E}, journal = {Toxicology mechanisms and methods}, doi = {10.1080/15376510903280107}, volume = {19}, number = {8}, issn = {1537-6524}, title = {Lindane-induced cytotoxicity and the role of vitamin E in Chinese hamster ovary (CHO-K1) cells}, keyword = {CHO-K1 cells, cytotoxicity, IC50 values, lindane, vitamin E} }

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