Napredna pretraga

Pregled bibliografske jedinice broj: 394335

The assessment of genotoxic effects of wastewater from a fertilizer factory


Durgo, Ksenija; Oreščanin, Višnja; Lulić, Stipe; Kopjar, Nevenka; Želježić, Davor; Franekić Čolić, Jasna
The assessment of genotoxic effects of wastewater from a fertilizer factory // JAT. Journal of applied toxicology, 29 (2009), 1; 42-51 doi:10.1002/jat.1381 (međunarodna recenzija, članak, znanstveni)


Naslov
The assessment of genotoxic effects of wastewater from a fertilizer factory

Autori
Durgo, Ksenija ; Oreščanin, Višnja ; Lulić, Stipe ; Kopjar, Nevenka ; Želježić, Davor ; Franekić Čolić, Jasna

Izvornik
JAT. Journal of applied toxicology (0260-437X) 29 (2009), 1; 42-51

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Phosphogypsum ; Ames assay ; lipid peroxidation ; DNA damage ; comet assay

Sažetak
In this study we investigated cytotoxic, mutagenic and genotoxic effects of different concentrations of wastewater from the phosphoric gypsum depot near the factory for fertilizing agents INA Petrokemija (Kutina, Croatia). The Ames test was performed on Salmonella typhimurium TA98 and TA100 strains, in the presence of S9 mix, glutathione and buffer, respectively. Cytotoxicity was studied on human laryngeal carcinoma cells (HEp2) and human cervical cells (HeLa). The level of lipid peroxidation in these two cell lines was evaluated in parallel. To establish the levels of primary DNA damage, the alkaline comet assay was performed on treated human peripheral blood leukocytes. No mutagenic effects of phosphoric gypsum on Salmonella typhimurium strains in the presence of S9 mix, GSH or PBS were observed. However, strong cytotoxic effect was observed on both human cell lines when they were treated with different concentrations of wastewater. Lipid peroxidation was induced and increased by prolonged time of incubation, highlighting that the damage was not repaired, but increased with the time of incubation. The results of the alkaline comet assay indicate significant DNA damaging potential of wastewater for human leukocytes. Since phosphoric gypsum transport water in its present composition and acidity is highly toxic and acts as prooxidant, causing free radicals formation and DNA damage, urgent neutralization/purification of the wastewater to a level acceptable for disposal into the environment is mandatory. Copyright © ; ; 2008 John Wiley & Sons, Ltd.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biotehnologija



POVEZANOST RADA


Projekt / tema
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
058-0582261-2246 - Utjecaj mutagena i antimutagena na molekularne procese u stanici (Reno Hrašćan, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati