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Citotoxicity Status of Electroplating Wastewater prior/after Neutralization/Purification with Alkaline Solid Residue of Electric Arc Furnace Dust


Oreščanin, Višnja; Kopjar, Nevenka; Durgo, Ksenija; Elez, Loris; Findri Guštek, Štefica; Franekić Čolić, Jasna
Citotoxicity Status of Electroplating Wastewater prior/after Neutralization/Purification with Alkaline Solid Residue of Electric Arc Furnace Dust // Journal of Environmental Science and Health Part A, 44 (2009), 3; 273-278 doi:10.1080/10934520802597945 (međunarodna recenzija, članak, znanstveni)


Naslov
Citotoxicity Status of Electroplating Wastewater prior/after Neutralization/Purification with Alkaline Solid Residue of Electric Arc Furnace Dust

Autori
Oreščanin, Višnja ; Kopjar, Nevenka ; Durgo, Ksenija ; Elez, Loris ; Findri Guštek, Štefica ; Franekić Čolić, Jasna

Izvornik
Journal of Environmental Science and Health Part A (1093-4529) 44 (2009), 3; 273-278

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Electroplating wastewater ; alkaline solid residue ; MTT assay ; HeLa cells ; HEp2 cells ; alkaline comet assay

Sažetak
Toxicological safety of new procedure for the neutralisation/purification of wastewater originated from zinc plating facility was investigated. Wastewater was treated with alkaline solid residue-by-product of zinc recovery from electric arc furnace dust. For determination of citotoxic potential of untreated and purified wastewater MTT test on HEp2 (human laryngeal carcinoma) and HeLa (human cervical carcinoma) cells lines and alkaline comet assay on human leukocytes were used. 100% of the sample as well as different dilutions were tested. Compared to negative control 100, 75 and 50 % of the sample of untreated wastewater significantly decreased survival of both HEp2 and HeLa cell lines. In the presence of undiluted sample survival percentage of HeLa and HEp2 cells were only 2.3 and 0.3 % respectively. Only undiluted purified wastewater showed slight but insignificant decrease of the survival of both cell lines. Even 0.5 % of the sample of original electroplating wastewater exhibited significantly higher value of all comet assay parameters compared to negative control. There was no significant difference between negative control and purified wastewater for any of comet assay parameters. Significantly lower level of primary DNA damage recorded after treatment with purified water, even comparable with negative control, confirmed effectiveness of the purification process.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biotehnologija



POVEZANOST RADA


Projekt / tema
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
058-0582261-2246 - Utjecaj mutagena i antimutagena na molekularne procese u stanici (Reno Hrašćan, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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